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Monday, August 3, 2020 | History

2 edition of Thin-layer chromatography of selected cardiac glycosides. found in the catalog.

Thin-layer chromatography of selected cardiac glycosides.

Dennis A. Oeth

Thin-layer chromatography of selected cardiac glycosides.

by Dennis A. Oeth

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Published by Loras College. Wahlert Memorial Library .
Written in English


Edition Notes

B. Sc. thesis, Loras College, Dulouque, Iowa, 1966.

The Physical Object
Pagination35p.
Number of Pages35
ID Numbers
Open LibraryOL13694837M

Thin-layer chromatography of steroids Szepesi and Gazdag wrote a book chapter on the TLC of steroids, and it included information on sample preparation as well as stationary-phase and mobile-phase systems useful for the separation of steroidal pharmaceuticals [2]. The authors also. After the perfusion, extracts of liver, bile and plasma were subjected to thin layer chromatography in order to detect the radioactively labelled glycosides and their metabolites. 2. The ouabain concentration in the plasma approached the equilibrium stage within 45 minutes.

  A densitometric reversed-phase thin-layer chromatographic(RP-TLC) method for the determination of digitalis glycosides in Digitalis purpurea leaves has been developed. The procedure involves extraction of dry leaf powder with ethanol/chloroform () and clean-up by Sep-Pak cartridges prior to RP-TLC analysis.   THIN LAYER CHROMATOGRAPHY GOKULAKRISHNAN TLC 1 2. GOKULAKRISHNAN TLC 2 INTRODUCTION TLC is a form of liquid chromatography consisting of: A mobile phase (developing solvent). A stationary phase (a plate or strip coated with a form of silica gel). Analysis is performed on a flat surface under atmospheric pressure and room temperature.

  Preliminary phytochemical screening was carried out on the extracts. Thin layer chromatography (TLC) was carried out on silica gel precoated plates in (hexane/ethyl acetate), (hexane/ethyl acetate), and (ethyl acetate/methanol) mobile phases for hexane, ethyl acetate and methanol extracts respectively. Thin-Layer Chromatography (TLC)-Bioautography. Of 20 plants leaves extracts tested for anticariogenic activity. Only one plant extracts was selected showing maximum growth of inhibition against SMU, L. casei and L. acidophilus were respectively selected for used for bioautography.


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Thin-layer chromatography of selected cardiac glycosides by Dennis A. Oeth Download PDF EPUB FB2

Separation of Cardiac Glycosides by Thin-Layer Chromatography. WHITE1 AND DENNIS 0ETH. Abstract. Two structurally similar cardiac glycosides, digoxin and digitoxin, and their aglycones, digoxigenin and digitoxigenin, were separated by thin-layer chromatography using glass plates coated with silica gel H.

Of 50 solvent. Research Articles Thin-layer Chromatography of Cardiac Glycosides Eugene J. Johnston, Allen L. Jacobs, Analytical Research Department, Sandoz Pharmaceuticals, Hanover, N. Analytical Research Department, Sandoz Pharmaceuticals Hanover N.

J A rapid thin-layer chromatographic procedure for the separation and identification of the common cardiac glycosides is by: 5. The Phytochemical and Thin Layer Chromatography Profile of cardiac glycosides, whereas alkaloids, saponins and carbohydrates were completely absent.

Similarly, the ethanol extract contained flavonoids, tannins, phenols and cardiac glycosides. The aqueous extract a book for drugs some of which have been used even today include Rhei. This second edition of Plant Drug Analysis includes more than updated color photographs of superb quality demonstrating chromatograms of all relevant standard drugs.

All drugs presented meet the standard of the official pharmacopoeia and originate from well-defined botanical sources.

With this guide the technique of thin layer chromatography can be easily used without previous 3/5(2). PDF | OnSreyorn Soeurn, Pich Srey, Pisal Lay, Srey Lin Heng, Chanlida Sovan, Sin Chea, Samell Keo published Phytochemical and Thin Layer Chromatography Analyses of.

Thin layer chromatography can be used to identify natural products like essential oils or volatile oil, fixed oil, glycosides, waxes, alkaloids, etc It is widely used in. Cardiac glycosides acts on contractile force of cardiac muscles that can interfere in functioning of heart and hence acts as toxic agents.

Some of them have been recommended for treatment of uncontrolled heart conditions after purification and modification. Ouabain () (Fig. ), is a cardiac glycoside extracted from ripe seeds of Strophanthus gratus and bark of Acokanthera ouabaio, used. Paper Chromatography and Electrophoresis, Volume II presents methods, techniques and complete experimental procedures in paper chromatography.

The book provides information and applications of paper chromatography such as the theory, mechanism, and fundamentals of the process; the separation of amino acids, carbohydrates, lipophilic steroids.

Quercetin was found to be % w/w and % w/v by high performance thin layer chromatography (HPTLC) and high-pressure liquid chromatography (HPLC) respectively.

After preliminary analysis by thin layer chromatography for the presence of bioactive compounds, crude extracts of five fungal cultures were selected for HPLC. HPLC chromatograms revealed the production of glycoside digoxin from the five selected endophytic cultures, thus providing a novel, alternative and eco-friendly source for the production.

Purchase Paper and Thin Layer Chromatography - 4th Edition. Print Book & E-Book. ISBNThin-layer chromatography (TLC) was performed on silica gel plates coated with a uorescent indicator (Merck, Kieselgel 60 F ) by eluting with the resolving mixture hexane=ethyl-acetate (3: 7.

Behaviour of cardiac glycosides and cardenolides related to digitoxigenin on sephadex LH Journal of Chromatography A(1), DOI: /S(00)   Journal of Chromatography, () (D Elsevier Scientific Publishing Company, Amsterdam - Printed in The Netherlands C11iROM.

10, Note High-speed liquid chromatography of cardiac glycosides in milkweed plants and monarch butterflies JANET M- ENSON and JAMES N- SEWER Department of Environmental Toxicology, University of California Dasis, Calif. Cardiac glycosides were present in all the extracts of both root and stem bark.

Cardiac Glycosides have been reported to be highly toxic at high concentration, but has some pharmacological uses. (Japan) (Received August 5th, ) The cardiac glycosides are therapeutically important substances which are widely used in the treatment of heart disease.

Methods published so far for the determination of digitalis glycosides are based on paper, thin-layer and gas-liquid chromatography. Scilliroside and other bufadienolide glycosides in red squill, Urginea maritima (L.) Baker, were identified by isolation, high-performance liquid chromatography and thin-layer chromatography.

Definition: • Thin layer chromatography (TLC) is an important technique for identification and separation of mixtures of organic compounds. It is useful in: • Identification of components of a mixture (using appropriate standards) • analyzing fractions collected during purification.

• analyzing the purity of a compound. A procedure is described for extracting cardiac glycosides and their aglycones from dried leaf powder of Digitalis purpureaL. by a water-ethanol gradient elution followed by Soxhlet ram amounts of pure digitoxin had been added to the leaf powder for studying its effects on the solubilities and removal of interfering plant pigments and on the recovery of steroidal substances by.

Cardiac glycosides (CG) are of forensic importance because of their toxicity and the fact that very limited methods are available for identification of CG in biological samples.

In this study, we have developed an identification and quantification method for digoxin, digitoxin, deslanoside, digoxigenin, and digitoxigenin by high-performance liquid chromatography tandem mass. Preface to the Second Edition More than 12 years have passed since the first and very successful attempt was made to reproduce the thin layer chromatography (1'LC) separation of medicinal plant drugs in the form of color TLC fingerprints in a book.This book, intended for pharmacists, chemical and pharmaceutical researchers and technicians, drug importers and exporters, governmental chemical control agencies and health authorities, is a useful reference for analysing plant drugs by TLC, identifying unknown drugs and monitoring the purity or constituents of a given drug.

There are 19 chapters: (1) Alkaloid drugs; (2) Drugs containing. The petroleum ether extract was subjected to thin layer chromatography about ml of conc.

Pet. ether extract was loaded on the plate by using capillary tube. During spotted plates were.